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dc.contributor.advisor Mercer, Frances en
dc.contributor.author Moran, Jose en
dc.date.accessioned 2020-05-21T23:31:43Z en
dc.date.available 2020-05-21T23:31:43Z en
dc.date.issued 2020-05-21 en
dc.identifier.uri http://hdl.handle.net/10211.3/215968 en
dc.description.abstract Trichomonas vaginalis (Tv) is a parasite that causes the most common non-viral sexually transmitted infection worldwide, and annually infects 3.7 million people in the United States. Tv is associated with fertility and pregnancy issues, such as infertility, spontaneous abortion, and cervical cancers. Vaginal smear samples have shown high levels of immune cells called neutrophils in Tv-infected women. Neutrophils have been shown to swarm Tv and “nibble” the parasite to death. This “nibbling” is called trogocytosis and has been shown to be contact-dependent, however the molecules mediating contact between neutrophils and Tv are unknown. Opsonins are proteins that circulate in blood and attach to the surfaces of pathogens, signaling for destruction. It has been shown that the opsonin iC3b can bind to Tv and bind to surface receptors on neutrophils such as complement receptor 4 (CR4), therefore we hypothesize that iC3b crosslinks Tv to neutrophils via CR4. We believe that the deletion of CR4 in PLB 985 cells will decrease the trogocytic activity of the resultant NLCs, and thus their ability to kill Tv. CR4 is made of two subunits, CD11c and CD18. In this study, we showed that both subunits are present in neutrophil-like cells (NLCs), a suitable model for studying trogocytic killing of Tv as primary neutrophils are short-lived. Using a progenitor cell line that can be differentiated into neutrophil-like cells, we have shown the generation of a heterogenous CD11c-knockout cell population using CRISPR/Cas9 gene editing. This study shows that CD11cknockouts were achieved and that experiments determining the role of CD11c in the trogocytic killing of Tv parasites by neutrophils are possible. Determining the role of CR4 in neutrophil trogocytosis of Tv, will better characterize this novel antimicrobial process, which may better inform novel preventative Tv therapies. Current trichomoniasis treatments are non-specific antibiotics that are used to cure infection, though antibiotic resistance against that will protect the many people who are infected annually, especially women as they suffer more severe health outcomes due to Tv infection. en
dc.format.extent 34 pgs. en
dc.language.iso en en
dc.publisher California State Polytechnic University, Pomona en
dc.rights.uri http://www.cpp.edu/~broncoscholar/rightsreserved.html en
dc.subject sexually transmitted infection en
dc.subject non-viral sexually transmitted infection en
dc.subject S.T.I. en
dc.title The Potential Role of Complement Receptor 4 in the Trogocytic Killing of Trichomonas vaginalis by Neutrophil-Like Cells en
dc.type Student Research en
dc.contributor.department Department of Biological Sciences en
dc.rights.license All rights reserved en

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