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dc.contributor.advisor Mercer, Frances en
dc.contributor.author Leka, Aljona en
dc.contributor.author Moran, Jose en
dc.date.accessioned 2020-05-21T23:31:38Z en
dc.date.available 2020-05-21T23:31:38Z en
dc.date.issued 2020-05-21 en
dc.identifier.uri http://hdl.handle.net/10211.3/215967 en
dc.description.abstract Trichomonas vaginalis (T. vaginalis) causes the non-viral sexually transmitted infection (STI), trichomoniasis. Trichomoniasis affected almost 276.4 million people globally in 2008 alone, with most incidents occurring in underserved communities. The main curative treatment for T. vaginalis is an antibiotic, Metronidazole, though antibiotic resistance is on the rise. Neutrophils are the first responders against T. vaginalis, killing the parasite through a recently discovered process called trogocytosis, in which the neutrophils “nibble” on the parasite’s membrane leading to killing of the parasite. However, current literature lacks the knowledge of which molecular components are involved in trogocytosis of T. vaginalis. Trogocytosis is a contact-dependent process mediated through opsonins, specifically iC3b, which serves as a tag to make the pathogens “tasty” for the neutrophils. Complement receptor (CR) 3, which is composed of dimer CD11b/CD 18, is known to bind iC3b leading to phagocytosis, but has yet to be correlated with trogocytosis. We hypothesize that CR3 plays a role in mediating trogocytosis of T. vaginalis. To test our hypothesis, we will knockout CR3 on neutrophil like cells using, CRISPR-Cas9 and test whether the CR3 knockout cells are able to kill the parasite with the same efficiency as the unmutated cells, when cultured with T. vaginalis through a cytotoxicity assay. en
dc.format.extent 25 pgs. en
dc.language.iso en en
dc.publisher California State Polytechnic University, Pomona en
dc.rights.uri http://www.cpp.edu/~broncoscholar/rightsreserved.html en
dc.subject sexually transmitted infection en
dc.subject non-viral sexually transmitted infection en
dc.subject S.T.I. en
dc.title Investigating the role of CR3 in trogocytosis of Trichomonas vaginalis cells by Neutrophil-like cells en
dc.type Student Research en
dc.contributor.department Department of Biological Sciences en
dc.rights.license All rights reserved en

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